Friday, February 28, 2020

Nicoletta Sacchi

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Nicoletta Sacchi

FlyingFoxBoi: ←Created page with 'Nicoletta Sacchi is an Italian professor of oncology at the Roswell Park Comprehensive Cancer Center. She is the co-discoverer of the Acid gu...'


Nicoletta Sacchi is an [[Italy|Italian]] professor of [[oncology]] at the [[Roswell Park Comprehensive Cancer Center]]. She is the co-discoverer of the [[Acid guanidinium thiocyanate-phenol-chloroform extraction]] method to [[RNA extraction|extract RNA]] from biological samples with [[Trizol]].<ref name=":0"></ref> As a consequence of this groundbreaking discovery she is now considered the most [[cited]] female scientist in the world.<ref name=":1">Liquid error: wrong number of arguments (given 1, expected 2)</ref>

== Career ==
Sacchi studied for her PhD at the [[University of Milan]], investigating the [[genetics]] of [[yeast]], and she remained within the fields of genetics for her postdoctoral training at the same University and [[Erasmus University Rotterdam|Erasmus University]] [[Rotterdam]].<ref name=":0" /> Sacchi worked on the generation of [[Antibody|antibody repertoire]]<nowiki/>s by the [[Terminal deoxynucleotidyl transferase|TdT]] [[DNA polymerase]] in the lab of [[Georges J. F. Köhler|Georges Köhler]] at the [[Basel Institute for Immunology]].<ref name=":0" /> She moved the US and switched to cancer research, focussing on the analysis of the genetics of patient samples. This required a high yield technique to extract RNA, so her and colleague Piotr Chomczynski developed a new method to extract the nucleus acid.<ref>Liquid error: wrong number of arguments (given 1, expected 2)</ref> Since the publication of the technique in 1987 it has become ubiquitous in biological research, earning the paper the accolade of being one of the most cited in history.<ref name=":1" /><ref>Liquid error: wrong number of arguments (given 1, expected 2)</ref>

== References ==

February 28, 2020 at 01:23PM

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